Strand Displacement during Deoxyribonucleic Acid Synthesis at Single Strand Breaks
نویسندگان
چکیده
منابع مشابه
Strand displacement during deoxyribonucleic acid synthesis at single strand breaks.
Synthesis of DNA by DNA polymerases has been studied by using circular duplex DNA templates which contain single phosphodiester bond interruptions (nicks). Escherichia coli DNA polymerase can initiate synthesis at nicks by the covalent attachment of nucleotides to the 3’ end of the primer strand. An initial phase of synthesis is accompanied by the removal of nucleotides from the 5’ end of the n...
متن کاملEnzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid.
Pancreatic DNase has been used to introduce single phosphodiester bond interruptions (nicks) into double stranded circular DNA. Such DNA substrates have been used to study the action of several exonucleases at the site of nicks. Escherichia coli exonuclease III and the exonuclease activities of phage T4 DNA polymerase (3’ + 5’nuclease) and E. coli DNA polymerase (3’ --f 5’and 5’ + 3’nuclease) i...
متن کاملEnzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.
Double stranded DNA serves as a template primer for Escherichia coli DNA polymerase when the DNA contains a single strand break (a nick) with a 3’-hydroxyl terminus. Initiation of replication entails covalent extension of the 3’-hydroxyl terminus and a concurrent 5’ + 3’ nuclease action by the enzyme. The primer strand is hydrolyzed at the 5’ side of the nick while the synthetic activity cataly...
متن کاملThymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli.
Single-strand breaks, as measured by the McGrath and Williams procedure, occur in chromosomal deoxyribonucleic acid of Escherichia coli cells during thymine starvation.
متن کاملSingle-strand interruptions in replicating chromosomes cause double-strand breaks.
Replication-dependent chromosomal breakage suggests that replication forks occasionally run into nicks in template DNA and collapse, generating double-strand ends. To model replication fork collapse in vivo, I constructed phage lambda chromosomes carrying the nicking site of M13 bacteriophage and infected with these substrates Escherichia coli cells, producing M13 nicking enzyme. I detected dou...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62341-5